Table 1.
The effect of alisporivir treatment on the parameters of respiration and oxidative phosphorylation of skeletal muscle mitochondria of studied groups of mice.
| Animal (n = 10) | State 2 | State 3 | State 4 | State 3UDNP | RC | ADP/O |
|---|---|---|---|---|---|---|
| nmol O2/min per 1 mg of Protein | Relative Units | |||||
| WT | 19.6 ± 1.0 | 172.7 ± 6.7 | 26.2 ± 1.1 | 213.4 ± 10.7 | 6.6 ± 0.1 | 3.1 ± 0.3 |
| WT+Ali | 17.9 ± 0.8 | 156.4 ± 7.1 | 23.0 ± 0.6 | 202.0 ± 12.5 | 6.8 ± 0.2 | 2.9 ± 0.1 |
| mdx | 17.1 ± 1.3 | 127.9 ± 10.0 * | 25.1 ± 1.3 | 150.4 ± 13.2 * | 5.1 ± 0.2 * | 3.2 ± 0.2 |
| mdx+Ali | 21.0 ± 0.8 | 164.4 ± 3.3 | 28.7 ± 1.3 | 201.9 ± 7.6 | 5.8 ± 0.2 *# | 2.9 ± 0.1 |
Medium composition: 120 mM KCl, 5 mM NaH2PO4, and 10 mM HEPES-KOH buffer (pH 7.4). Respiration of mitochondria was fueled by 2.5 mM glutamate + 2.5 mM malate. Respiration of mitochondria in state 3 was initiated by 200 μM ADP. The data are presented as means ± SEM. * p < 0.05 versus other groups. # p < 0.05 versus mdx group.