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. 2021 Sep 2;10(9):1410. doi: 10.3390/antiox10091410

Figure 4.

Figure 4

Annexin V status of EANT-treated leukemia cells. (A,B) Histogram and statistical analysis for annexin V expression. Apoptosis (%) was calculated to the populations of annexin V (+)/7AAD (+/−) (%). HL-60 cells were treated with EANT (24 h, 0 to 4 μg/mL). The control contained 0 μg/mL EANT but 0.1% DMSO. (C,D) Histogram and statistical analysis for annexin V detection of HL-60 cells following NAC/EANT treatment. NAC/EANT indicates that cells are either pretreated with NAC (5 mM, 1 h) or post-treated with EANT (0 and 4 μg/mL for 0, 12, and 24 h). (E) Caspase 3/7 activity assay of HL-60, K-562, and MOLT-4 cells following NAC/EANT treatment for 1 h/24 h. Data, mean ± SD (n = 3). Data showing different letters at the top revealed a significant difference (p < 0.05–0.0001 for multiple comparisons). (F) Western blot analysis of c-PARP and c-Cas 3 expressions in HL-60 cells following NAC (5 mM, 1 h)/EANT or ZVAD (100 μM, 2 h)/EANT treatments.