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. 2021 Sep 2;10(9):1410. doi: 10.3390/antiox10091410

Figure 5.

Figure 5

ROS content of EANT-treated leukemia cells. (A,B) Histogram and statistical analysis for ROS content. (+) at the right side of each panel is regarded as the population of ROS (+) (%). HL-60 cells were treated with EANT (1 h, 0 to 4 μg/mL). The control contained 0 μg/mL EANT but 0.1% DMSO. (C,D) Histogram and statistical analysis for ROS content of HL-60 cells following NAC/EANT treatment. NAC/EANT indicates that cells are either pretreated with NAC (5 mM, 1 h) or post-treated with EANT (0 and 4 μg/mL for 0 to 60 min). Data, mean ± SD (n = 3). Data showing different alphabets at the top reveal a significant difference (p < 0.05–0.0001 for multiple comparisons).