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. 2021 Sep 2;10(9):1410. doi: 10.3390/antiox10091410

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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8-OHdG content of EANT-treated leukemia cells. (A,B) Histogram and statistical analysis for 8-OHdG content. (+) on the right side of each panel is regarded as the population of 8-OHdG (+) (%). HL-60 cells were treated with EANT (1 h, 0 to 4 μg/mL). The control contained 0 μg/mL EANT but 0.1% DMSO. (C,D) Histogram and statistical analysis for 8-OHdG content of HL-60 cells following NAC/EANT treatment. NAC/EANT indicates that cells were either pretreated with NAC (5 mM, 1 h) or post-treated with EANT (0 and 4 μg/mL for 0, 12, and 24 h). Data, mean ± SD (n = 3). Data showing different letters on the top reveal a significant difference (p < 0.0001 for multiple comparisons).