Skip to main content
. 2021 Sep 10;13(18):4552. doi: 10.3390/cancers13184552

Figure 1.

Figure 1

FAP regulates MT2A expression upstream in CAF-like cells. (A,B). The expression of MT2A mRNA and MT2A protein in mesenchymal stem cells (MSCs), CAF8, CAF9, and CAF15 cells was detected by quantitative real-time-PCR (qRT-PCR) (A) and Western blotting (B). After Western blotting, the normalized relative expression fold-change were calculated using the ImageJ software, and the values were arbitrarily set as 1.00 for control MSCs. (C–E). qRT-PCR (C,E) and Western blotting (D) to confirm FAP knockdown and change in the expression of MT2A. The three types of CAF-like cells were transfected with siRNA targeting FAP (siFAP) and the negative control siRNA (siNC). After Western blotting, the normalized relative expression fold-change were calculated using the ImageJ software, and the values were arbitrarily set as 1.00 for cells transfected with control siNC. Data are presented as mean ± SEM (** p < 0.01, *** p < 0.001).