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. 2021 Sep 10;22(18):9799. doi: 10.3390/ijms22189799

Figure 4.

Figure 4

Using NGS to investigate miRNAs released by MTs treated with MTX and APAP. Cell culture supernatant from MTs exposed to MTX and APAP for 10 days were collected to perform miRNA analysis using NGS. PCA analysis of differentially released miRNAs was carried out using R as described in methods section (A). NGS demonstrated that MTX and APAP differentially released miRNAs. Venn diagram results show that release was increased for 57 and decreased for 225 common miRNAs between the treatments (B). Four of the MTX-specific miRNAs were chosen based on the literature search and volcano plots for MTX (C) and APAP (D) demonstrate the release of the four miRNAs (miR-199a-5p, mIR-214-3p, miR-125a-5p and miR-99b-5p) is significantly increased by MTX treatment but not APAP.