Table 2.

Reagent quantities for miRNA mimic transfection.

Plate Size	Cell Number	Concentration (mM)	HiPerfect Reagent	Mimic	DMEM without Supplements	Final Medium + Reagent Quantity per Well
96-well	2000	40	0.75 µL	0.12 µL	24.25 µL	200 µL
		80	0.75 µL	0.24 µL	24.25 µL
48-well	10,000	40	1.5 µL	0.24 µL	48.5 µL	400 µL
		80	1.5 µL	0.48 µL	48.5 µL
6-well	60,000	40	6 µL	0.96 µL	194 µL	1600 µL
		80	6 µL	1.92 µL	194 µL