Figure 2.

Niclosamide and pyrvinium do not induce cell death at non-cytotoxic nM concentration in osteosarcoma cells. (A) Representative images of colonies in MG-63 and SAOS-2 cells against niclosamide and pyrvinium at the indicated concentration. Osteosarcoma cells treated with 0.125, 0.25, and 0.5 μM niclosamide and 0.01, 0.03, and 0.1 μM pyrvinium for 72 h and then stained with 0.25% crystal violet after colony formation. (B) The colony number of osteosarcoma cells treated with niclosamide and pyrvinium. Colonies of more than 50 cells were counted after crystal violet staining. For all of the above assays, each assay condition was evaluated in triplicate. Results are expressed as the mean ± SD (error bars; n = 3, n.s., not significant). (C) The survival curve of osterosarcoma cells treated with niclosamide and pyrvinium for 72 h.