Figure 4.

Effect of 4f and 4m on LPS-induced c-Jun (Ser63) phosphorylation. Human MonoMac-6 monocytic cells were pretreated with indicated concentrations of 4f or 4m for 30 min, followed by treatment with LPS (250 ng/mL) or control vehicle (1% DMSO) for another 30 min. The cells were lysed, and the lysates were analyzed by Western blotting. Total JNK (non-phosphorylated) was used as loading control for the lysates. A representative blot from two independent experiments is shown (Panel A). The blots were analyzed by densitometry, and the ratio of phospho-c-Jun/total c-Jun is shown in Panel B.