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. 2021 Sep 18;11(9):2728. doi: 10.3390/ani11092728

Figure 2.

Figure 2

Example of the analysis of the tissue slices in the QuPath software. After importing the scanned images, a grid was placed on the pictures. For each tissue section, four squares of 500 µm × 500 µm were selected: two squares in a fibrotic region (region 1 and 2, green) and two squares in an inflammation region (region 3 and 4, red). On each of these four squares the positive pixel count algorithm was run. Pixels that were counted as positive pixels are encircled in yellow (insert).