Figure 4.

MAC387 staining. (A): Representative pictures of immunohistochemical stainings showing the differences in MAC387 immunolabeling between the inflammatory and fibrotic regions in EGT and physiological healing in experimental wounds at 6 days (d6) or 19 days (d19) post-injury. Scale bar = 100 µm. (B,C): MAC387 staining was quantified in sections of horse wound tissue in the inflammatory regions (B) and in the fibrotic regions (C). An unpaired Kruskal–Wallis was performed to detect the differences between the different groups, i.e., EGT wounds, d6 and d19. A pairwise comparison was then made between the different groups with the aid of an unpaired Wilcoxon test. A significant difference was detected between EGT-developing wounds and physiological healing wounds of 19 days in both the fibrotic and inflammatory regions of the wound. A p-value of <0.05 was considered significant. Green circles represent horses with an age between 7 and 15 years (age range of the experimental horses) and red triangles represent younger horses. See also Figure S3 for the unprocessed pictures.