Table 2.
Molecular weight (kDa) of gelatin from turbot skin in distributions shown in Figure 1. Rt: retention time; Mw: weight average molecular weight; Mn: number average molecular weight; PDI: polydispersity index. Peak area (%) corresponds to refractive index detector. Values are represented as mean ± standard deviations (n = 2), except for M4.
Method | Peak Number | Rt (min) | Mw (kDa) | PDI | Peak Area (%) |
---|---|---|---|---|---|
M1 | 1-high Mw | 37.6–44.3 | - | - | 7.9 ± 0.3 |
2 | 45.3 ± 0.2 | 202.7 ± 0.5 | 1.044 | 31.5 ± 0.6 | |
3 | 48.4 ± 0.0 | 107.1 ± 2.6 | 1.023 | 32.0 ± 1,3 | |
4-low Mw | 50.3–65.2 | <100 | - | 28.6 ± 0.4 | |
M2 | 1-high Mw | 37.7–44.5 | - | - | 4.7 ± 0.2 |
2 | 45.6 ± 0.0 | 208.1 ± 2.9 | 1.021 | 15.3 ± 0.9 | |
3 | 47.6 ± 0.0 | 150.3 ± 1.3 | 1.004 | 8.4 ± 018 | |
4 | 48.4 ± 0.0 | 111.6 ± 0.7 | 1.012 | 20.7 ± 0.9 | |
5-low Mw | 48.4–67.4 | <100 | - | 50.9 ± 3.7 | |
M3 | 1-high Mw | 36.9–44.7 | - | - | 3.8 ± 0.3 |
2 | 45.6 ± 0.0 | 214.6 ± 4.3 | 1.024 | 11.5 ± 2.7 | |
3 | 47.5 ± 0.0 | 154.1 ± 1.8 | 1.004 | 6.1 ± 0.7 | |
4 | 48.4 ± 0.0 | 113.5 ± 0.7 | 1.011 | 21.7 ± 1.3 | |
5-low Mw | 49.3–6.6 | <100 | - | 57.0 ± 5.0 | |
M4 | 1-high Mw | 47.5 | 200.8 | 1.065 | 7.6 |
2 | 48.9 | 104.5 | 1.021 | 20.7 | |
3-low Mw | 49.9–70.0 | <100 | - | 71.72 | |
M5 | 1-high Mw | 36.0–44.9 | - | - | 0.6 ± 0.0 |
2 | 47.6 ± 0.1 | 200.4 ± 14.6 | 1.085 | 5.9 ± 4.2 | |
3 | 48.9 ± 0.4 | 113.1 ± 1.2 | 1.007 | 10.5 ± 3.1 | |
4-low Mw | 49.1–69.8 | <100 | - | 83.0 ± 7.4 | |
M6 | 1-high Mw | 37.0–44.6 | - | - | 1.1 ± 1.0 |
2-low Mw | 45.3–70.4 | <100 | - | 98.9 ± 1.0 | |
M7 | 1-high Mw | 34.5–46.4 | - | - | 0.6 ± 0.2 |
2-low Mw | 45.7–70.3 | <100 | - | 99.4 ± 0.2 |