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. 2021 Aug 27;10(9):2214. doi: 10.3390/cells10092214

Figure 2.

Figure 2

Cell cycle regulators are highly expressed during the first trimester of gestation. (A–C) Evaluation of positive cells in first trimester placental sections (n = 6; named 6–9 week), early-onset control (n = 20; named 25–33 week), and late-onset control samples (n = 10; named 34–40 week). The results are presented as bar and scatter plots showing the mean value ± SD. (A) Quantification of p21 (left panel), p–p21 (middle panel), and p27 (right panel) positive CTBs in %. (B) Quantification of p21 (left panel), p-p21 (middle panel), and p27 (right panel) positive stained STB area in %. (C) Quantification of p21 (left panel), p–p21 (second panel), p27 (third panel), and p57 (right panel) positive fCTBs in %. (D) The relative amount of the gene levels was analyzed from placental tissues: left panel CDKN1A (p21), middle panel CDKN1B (p27), and right panel CDKN1C (p57). The results are presented as relative quantification (RQ) with minimum and maximum range. TBP was used as the endogenous control. Unpaired Student’s t-test or Mann–Whitney U test referring to first trimester samples was used for statistical analysis, * p < 0.05, ** p < 0.01, *** p < 0.001. CTBs, cytotrophoblasts; fCTBs, cytotrophoblasts ongoing to fuse; STB, syncytiotrophoblast.