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. 2021 Aug 27;10(9):2214. doi: 10.3390/cells10092214

Figure 4.

Figure 4

p21 expression is reduced in early-onset PE complicated by the HELLP syndrome. (A–C) Quantification of cell cycle regulators in placental sections of control donors (control, n = 17) and placental tissues from early-onset PE complicated by the HELLP syndrome (ePEH, n = 16) using the H-score method. The results are presented as bar and scatter plots showing the mean value with SD. The percentage of positive stained cells/area is shown under each graph. (A) H-score of p21 (left panel), p–p21 (middle panel), and p27 (right panel) for CTBs. (B) H-score of p21 (left panel), p–p21 (middle panel), and p27 (right panel) for the STB area. (C) H-score of p21 (left panel), p–p21 (second panel), p27 (third panel), and p57 (right panel) for fCTBs. (D) Quantification of p21 positive (left panel), p–p21 positive (second panel), p27 positive (third panel), and p57 positive EVTs (right panel) in %. (E) Western blot analysis with extracts from placental tissues is shown. GAPDH served as the loading control. (F) The relative amount of the gene levels of CDKN1A (p21), CDKN1B (p27) and CDKN1C (p57) was analyzed with placental tissues. The results are presented as relative quantification (RQ) with minimum and maximum range. TBP was used as the endogenous control. Paired Student’s t-test or Wilcoxon-test was used for statistical analysis. CTBs, cytotrophoblasts; fCTBs, cytotrophoblasts ongoing to fuse; STB, syncytiotrophoblast; EVT, extravillous cytotrophoblasts; no, number; MV, mean value.