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. 2021 Sep 11;10(9):2389. doi: 10.3390/cells10092389

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Validation of transcriptome data by RT-PCR. Up- and downregulation of genes were confirmed for LDLR, StAR, ADCY, and HSD17B1; β-Actin was used as a control. Gel images of PCR products are shown for the young (A) and old (B) pregnancy and non-pregnancy groups. (C,D) Data of real-time RT-qPCR for up- and downregulated genes, based on NGS data from the pregnancy and non-pregnancy groups. Data are presented as means ± SEM of three replicates. Significant differences are indicated by asterisks (* p < 0.05).