Figure 3.

Growth of S. agalactiae s. III biofilm culture on coupons made from different materials in the CDC Biofilm Reactor System visualized with SYBR Green staining using confocal microscopy: (a) glass coupon; (b) polypropylene coupon; (c) polycarbonate coupon; (d) porous polycarbonate coupon. The reactor with full-strength broth was inoculated with overnight culture. After 24 h of incubation with mixing, a flow of 5×-diluted broth was started and continued for 24 h. Coupons were transferred to a glass-bottom plate, stained with SYBR Green, and viewed under a confocal laser scanning microscope.