Skip to main content
. 2021 Sep 14;22(18):9921. doi: 10.3390/ijms22189921

Table 2.

Gene-specific primers used in this study.

Name Sequences (5′–3′) Function
pK18_Adh3upF CGAGCTCGGTACCCGGGACGCCCTTTAACATGACCAG Construction of Adh3 mutant
pK18_Adh3downR AACGACGGCCAGTGCCACAGATGCGCTAAGGGAAAAC Construction of Adh3 mutant
pK18_Adh4upF CGAGCTCGGTACCCGGGTGCCCTTCATTATCAATTTCG Construction of Adh4 mutant
pK18_Adh4downR AACGACGGCCAGTGCCAGACATCGCTTTGAGTTGCAT Construction of Adh4 mutant
pK18_Adh6upF CGAGCTCGGTACCCGGGACGCACATTGGTCAGTTTTG Construction of Adh6 mutant
pK18_Adh6downR AACGACGGCCAGTGCCATTGCTGCAACCATAACAGGT Construction of Adh6 mutant
Adh1_rF TGTGATTGCCTGTGGTGAAT qRT-PCR
Adh1_rR ACACCGCCGTAAAGATGACT qRT-PCR
Adh2_rF GGTCGATTCATGCCGTACTT qRT-PCR
Adh2_rR TGTGGTAATACCCGCACAAA qRT-PCR
Adh3_rF TCAGTTACACCTGCCTATTCTTCA qRT-PCR
Adh3_rR CCCAAAGCCGACAATAACAT qRT-PCR
Adh4_rF TGCAAGATGAAGGGCTATTT qRT-PCR
Adh4_rR CACCGCCTAACGACACAATA qRT-PCR
Adh5_rF GCCAGCAGATAAAGCGGATT qRT-PCR
Adh5_rR TGTTGCCCCATATACATTACCA qRT-PCR
Adh6_rF TCTGGTGCACACAACCTACC qRT-PCR
Adh6_rR TCTAAAATCGCAGCATGTGG qRT-PCR
Adh7_rF GGCGAAAATATCGCAACAAT qRT-PCR
Adh7_rR ACCCAAACCACCAATACCAA qRT-PCR
pRsetB-His7-Peredox-mCherry_F GCCCTTTCGTCTTCAAGTAATACGACTCACTATAGGG Construction of peredox plasmid
pRsetB-His7-Peredox-mCherry_R AGCTGTCAAACATGAGTCACTTGTACAGTTCGTCCA Construction of peredox plasmid