Table 3.
Plasmids and bacterial strains used in this study.
| Plasmid | Description | Antibiotic Resistance (µg/mL) | Reference/Source |
|---|---|---|---|
| pK18mobsacB | Suicide vector for homologous recombination | Kan50 | [31] |
| pK18ΔAdh1 | pK18mobsacB contains the upstream and downstream regions of Adh1 | Kan50 | This study |
| pK18ΔAdh2 | pK18mobsacB contains the upstream and downstream regions of Adh2 | Kan50 | This study |
| pK18ΔAdh3 | pK18mobsacB contains the upstream and downstream regions of Adh3 | Kan50 | This study |
| pK18ΔAdh4 | pK18mobsacB contains the upstream and downstream regions of Adh4 | Kan50 | This study |
| pK18ΔAdh5 | pK18mobsacB contains the upstream and downstream regions of Adh5 | Kan50 | This study |
| pK18ΔAdh6 | pK18mobsacB contains the upstream and downstream regions of Adh6 | Kan50 | This study |
| pK18ΔAdh7 | pK18mobsacB contains the upstream and downstream regions of Adh7 | Kan50 | This study |
| pQE80L | Expression vector with colE1 origin for His-tag fusion protein purification | Amp50 | Qiagen |
| pAdh3 | Apr; Adh3 cloned into BamHI-HindIII sites of pQE80L | Amp50 | This study |
| pAdh4 | Apr; Adh4 cloned into BamHI-HindIII sites of pQE80L | Amp50 | This study |
| pAdh6 | Apr; Adh6 cloned into BamHI-SmaI sites of pQE80L | Amp50 | This study |
| pWH1266 | Apr; Tcr; shuttle vector for E. coli and A. baumannii | Amp50, Tc12.5 | [32] |
| pRsetB-His7tag- Peredox-mCherry | Apr; fluorescent biosensor of the cytosolic NADH/NAD+ redox state by combining a circularly permuted GFP T-Sapphire with a bacterial NADH-binding protein, Rex, and the red fluorescence of a tandemly attached mCherry. | Amp50 | [33,34] |
| pWH1266_peredox-mCherry | Apr; Tcr; permuted GFP T-Sapphire with a bacterial NADH-binding protein, Rex, and the red fluorescence of a tandemly attached mCherry from pRsetB-His7tag- Peredox-mCherry was cloned into EcoRI sites of pWH1266 | Amp50, Tc12.5 | This study |
| Strain | Description | Reference/Source | |
| E. coli DH5α | F−, supE44, hsdR17, recA1, gyrA96, endA1, thi-1, relA1, deoR, λ− | ATCC53868 | |
| Acinetobacter baumannii ATCC 19606 | Primary strain used in this study | [31] | |
| ∆Adh3(∆3) | Marker-less Adh3 deletion mutant | This study | |
| ∆Adh4(∆4) | Marker-less Adh4 deletion mutant | This study | |
| ∆Adh6(∆6) | Marker-less Adh6 deletion mutant | This study | |
| ∆Adh34(∆34) | Marker-less Adh34 double deletion mutant | This study | |
| ∆Adh36(∆36) | Marker-less Adh36 double deletion mutant | This study | |
| ∆Adh46(∆46) | Marker-less Adh46 double deletion mutant | This study | |
| ∆Adh346(∆346) | Marker-less Adh346 triple deletion mutant | This study | |
Amp: ampicillin; Kan: kanamycin.