Figure 1.

Effects of acute glucose shifts on cell viability and proliferative activity of THP-1 cells. (A,B) THP-1 cells were cultured separately in normal glucose (NG, 5.5 mM) and high glucose (HG, 25 mM). Some of them were exposed to a glucose shift (NG-to-HG or HG-to-NG). Lipopolysaccharide (LPS, 1 μg/mL) was used as a positive control. Cell viability and proliferation were assesses at the indicated incubation time points. Data are expressed as the mean ± SEM. The cell survival ratio is expressed as a percentage of the NG control.