Skip to main content
. 2021 Sep 15;22(18):9952. doi: 10.3390/ijms22189952

Figure 2.

Figure 2

Acute glucose shift induces the activation of the NLRP3 inflammasome in THP-1 cells. The media of THP-1 cells assigned as NG- and HG-cultured cells were changed into HG and NG media, as indicated. (A–C) The mRNA levels of NLRP3, caspase-1, and IL-1β were examined by real-time quantitative PCR. (D) Cell lysates were subjected to western blotting using antibodies against NLRP3, caspase-1, and IL-1β. THP-1 cells were pretreated with MCC950 (10 μM) for 2 h and then exposed to acute glucose shift for 24 h. For positive control, THP-1 cells were treated with LPS (1 μg/mL) for 4 h and with 5 mM ATP for the last 30 min. (E–G) The protein expression levels of NLRP3, caspase-1, and IL-1β were quantified using Image J software. (H,I) The levels of caspase-1 and IL-1β in the culture supernatant were determined using ELISA. Data are presented as the mean ± SEM from at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, ns, not significant.