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. 2021 Sep 15;22(18):9952. doi: 10.3390/ijms22189952

Figure 5.

Figure 5

Acute glucose shift-induced activation of the NLRP3 inflammasome is p38 MAPK, JNK /NF-κB-dependent. THP-1 cells were pretreated with SB203580 (10 μM), SP600125 (20 μM), and Bay 11-7082 (10 μM) for 2 h and then exposed to an acute glucose shift for 24 h. (A) Western blotting was performed to detect the expression of p-p38 MAPK, p-JNK, p-NF-κB, NLRP3, caspase-1, and IL-1β. (B–G) The expression levels of p-p38 MAPK, p-JNK, p-NF-κB, NLRP3, caspase-1, and IL-1β were quantified using ImageJ. (H) The level of IL-1β in the culture supernatant was determined by ELISA. Data are presented as the mean ± SEM from at least three independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001.