Figure 5.

The effect of tested eye drops on SIRC cell viability: (A) cell viability after incubation with eye drops containing choline salicylate; (B) results for eye drops containing choline salicylate and hyaluronic acid after storage at different conditions; (C) data for eye drops containing hyaluronic acid and sterilized at different conditions. Sterile 0.9% NaCl was used as a control. Moreover, an additional control—cell culture medium (M) only—was also used. Cell viability was measured by MTT assay immediately after incubation with tested solutions and 24 h after incubation. Moreover, the cell viability was measured using the NRU assay (24 h after incubation with tested eye drops). Data are presented as a mean value + SD from two independent experiments. Statistical significance (****) between groups was assessed by Dunnett’s multiple comparison (p < 0.0001).