Table 4.
Microbiological purity test results for eye drops with CS (2%) and HA (0.4%) stored in immediate packaging (minims) under controlled conditions (40 °C/75% RH).
| Time, Months | Sample | Sample Volume, mL |
Direct Inoculation | Membrane Filtration | ||
|---|---|---|---|---|---|---|
| TSB | TG | TSB | TG | |||
| 0 | 1 | Whole content—1 mL | No growth | No growth | No growth | No growth |
| 2 | Whole content—1 mL | No growth | No growth | No growth | No growth | |
| 3 | Half content—0.5 mL | No growth | No growth | No growth | No growth | |
| 3 | 1 | Whole content—1 mL | Growth | No growth | No growth | No growth |
| 2 | Whole content –1 mL | No growth | No growth | No growth | No growth | |
| 3 | Half content—0.5 mL | No growth | No growth | No growth | No growth | |
Note: TSB—tryptic soy broth; TG—fluid thioglycolate medium.