Figure 2.

Hand1 is upregulated upon Bmp4 overexpression (OE). (A,B) Whole-mount in situ hybridization of E9.5 Bmp4 OE embryo compared with control embryo; Bmp4 OE embryos expanded Hand1 expression in the SHF and SHF-derived OFT and RV. (C) qRT-PCR indicated increased Hand1 expression level in Bmp4 OE embryos compared with control embryos. e, eye; ba, branchial arch; oft, outflow tract; rv, right ventricle. Arrows and arrow heads point out in situ hybridization signals in the OFT and SHF. Data are presented as means ± s.e.m. * indicates p-value < 0.05.