Table 3.
Micronuclei induced by BDA in L5178Y cells.
| Treatment | Dose (µM) |
Cytotoxicity (%) |
MnBn Cells (‰) |
Total Micronuclei | Distribution of Micronuclei |
|||
|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 2 | 3 | |||||
| 4 h treatment | ||||||||
| Untreated | 0 | - | 8 | 16 | 1984 | 16 | - | - |
| BDA | 10 | 10 | 14 | 28 | 1972 | 28 | - | - |
| BDA | 15 | 23 | 22 *** | 44 | 1956 | 44 | - | - |
| BDA | 20 | 27 | 18 * | 36 | 1964 | 36 | - | - |
| 24 h treatment | ||||||||
| Untreated | 0 | - | 12 | 24 | 1976 | 24 | - | - |
| BDA | 10 | 49 | 20 | 40 | 1960 | 40 | - | - |
| BDA | 15 | 55 | 27 *** | 54 | 1947 | 52 | 1 | - |
| BDA | 20 | 62 | 36 *** | 75 | 1931 | 69 | 3 | - |
| MMC | 1.5 μg/mL | 2 | 24 *** | 51 | 1952 | 46 | 1 | 1 |
Cytotoxicity = 100–100 [(CBPIT-1)/(CBPIC-1)]; CBPIT = Cytokinesis-block proliferation index in treated cultures; CBPIC = Cytokinesis-block proliferation index in negative control cultures. MnBn, Micronucleated binucleated cells; * p < 0.05; *** p < 0.001; MMC, Mitomycin C.