Figure 2.

Maximum-likelihood phylogenetic tree to reveal relationships among Wolbachia strains and leafminer species. The phylogeny is inferred by IQTREE based on 3′ end COI sequences of leaf-mining species, and wsp sequences of Wolbachia detected from corresponding leaf-mining species. Published wsp sequences from other insect species were included to allocate the infections to Wolbachia supergroups (the Wolbachia wsp alleles in this study are labeled in bold, additional wsp alleles refer to Baldo et al. [52]). Numbers beside nodes are IQTREE ultrafast bootstrap and SH-aLRT values. ML bootstrap values > 60% are shown on branches.