Table 1.
Fitting parameters of the concentration-response relationship of zoledronic acid against native skeletal muscles fibers and primary bone cells and recombinant KATP channel subunits expressed in Hek293 cells line.
| Drugs Cell Types Patch Clamp Configuration |
Imax % | IC50 (M) | Slope |
|---|---|---|---|
| Zoledronic acid EDL fibers excised macropatches |
−100.1 ± 9.3 n = 17 fibers |
1.2 ± 1.4 ×10−6 | 0.38 ± 0.07 |
| Zoledronic acid SOL fibers excised macropatches |
−99.4 ± 5 n = 21 fibers |
* 2.1 ± 3.7 × 10−10 | 0.29 ± 0.05 |
| Zoledronic acid Femora primary bone cells Cell-attached patches |
−104.8 ± 8.7 n = 21 cells |
* 1.6 ± 2.8 × 10−10 | 0.31 ± 0.07 |
| Glibenclamide Femora primary bone cells cell-attached patches |
−88.32 ± 21 n = 13 cells |
* 5.07 ± 0.5 × 10−9 | 0.4 ± 0.08 |
| Glibenclamide Calvaria primary bone cells cell-attached patches |
−93 ± 14 n = 18 cells |
* 1.02 ± 0.6 × 10−8 | 0.5 ± 0.03 |
| Zoledronic acid Hek293 cells- KIR6.2-SUR2A Whole-cell |
−83.7 ± 1.2 n = 15 cells |
7.1 ± 3.1 × 10−6 |
* 0.12 ± 0.7 |
| Zoledronic acid Hek293 cells -KIR6.1-SUR2B Whole-cells |
−94 ± 4.8 n = 15 cells |
* 3.9 ± 2.7 × 10−10 | 0.59 ± 0.04 |
| Zoledronic acid Hek293 cells -KIR6.1-SUR1 Whole-cells |
* −40 ± 8.8 n = 3 cells |
/ | / |
| Zoledronic acid Hek293 cells -KIR6.2-SUR1 Whole-cells |
* −60.13 ± 4.1 n = 15 cells |
2.1 ± 4.1 × 10−5 |
* 0.18 ± 0.09 |
EDL, Extensor digitorum longus; SOL, Soleus. Whole-cell and cell-attached KATP currents were recorded in physiological condition, KATP currents in native fibers were recorded in excised macro-patches in the presence of symmetrical high K+ ions on both sides of the membrane patches. * Data significant difference vs. other groups by One WAY ANOVA with variance-ratio F > 1.621 for p < 0.05.