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. 2021 Aug 31;10(9):1111. doi: 10.3390/pathogens10091111

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Describing the protein separation and identification bySDS-PAGE and WB. The SDS-PAGE analysis of expression of rSAG1 using 12% acrylamide gel. Lane M shows protein marker (ThermoFisher Scientific, Life Technologies Co. Ltd., Zhongzheng, Taiwan, Cat# 10747-012), Lane 1 shows expression after 7 h of induction, Lane 2 shows expression after 4 h of induction, Lane 3 shows expression without induction, Lane 4 shows expression of competent BL21 culture after 7 h of induction (A). WB analysis of the rSAG1 using an Anti-6x His Tag Antibodies (ThermoFisher Scientific, Life Technologies Co. Ltd., Zhongzheng, Taipei city, Taiwan, Cat# MA1-21315). Lane M shows the protein marker—(Bio-Helix Co. Ltd. (GeneDirex), New Taipei city, Taiwan, Cat# PM007-0500), Lane 1 shows purified rSAG1 protein, Lane 2 shows induced control culture of cells without insertion of SAG1 (B).