Table 3.

Corresponding RNA copies/mL for all dilution steps based on RT-qPCR.

Dilution in DMEM
Virus Lineage	Initial Preparation Infectivity (TCID50/mL)	Units	1:10	1:100	1:1000	1:10,000	1:100,000
B.1.1 (Non-VOC)	4.28 × 105	RNA copies/mL	1.9 × 108	2.9 × 107	3.5 × 106	2.7 × 105	1.7 × 104
		Ct value E gene	15.7	18.7	22.0	26.1	30.4
B.1.1.7 (Alpha)	2.85 × 105	RNA copies/mL	2.0 × 108	2.2 × 107	2.9 × 106	3.7 × 105	5.1 × 104
		Ct value E gene	15.7	19.1	22.3	25.6	28.7
B.1.351 (Beta)	2.16 × 105	RNA copies/mL	2.6 × 108	3.1 × 107	3.4 × 106	5.2 × 105	6.9 × 104
		Ct value E gene	15.3	18.6	22.1	25.0	28.2
P.1 (Gamma)	1.77 × 105	RNA copies/mL	2.1 × 108	3.9 × 107	5.3 × 106	5.8 × 105	5.5 × 104
		Ct value E gene	15.6	18.2	21.4	24.8	28.6
B.1.617.2 (Delta)	4.64 × 104	RNA copies/mL	2.8 × 108	3.7 × 107	4.8 × 106	5.4 × 105	6.9 × 104
		Ct value E gene	15.1	18.3	21.5	25.0	28.2
Dilution in Saliva
B.1.1 (Non-VOC)	4.28 × 105	RNA copies/mL	2.2 × 108	2.4 × 107	2.6 × 106	2.7 × 105	2.7 × 104
		Ct value E gene	15.5	19.0	22.5	26.0	29.7
B.1.1.7 (Alpha)	2.85 × 105	RNA copies/mL	1.6 × 108	1.4 × 107	1.9 × 106	2.3 × 105	2.5 × 104
		Ct value E gene	16.0	19.9	23.0	26.3	29.8
B.1.351 (Beta)	2.16 × 105	RNA copies/mL	1.6 × 108	1.0 × 107	1.8 × 106	≈2 × 105 *	≈2 × 104 *
		Ct value E gene	16.0	20.3	23.1	N.A.	N.A.
P.1 (Gamma)	1.77 × 105	RNA copies/mL	2.1 × 108	2.4 × 107	3.1 × 106	3.9 × 105	4.1 × 104
		Ct value E gene	15.6	19.0	22.2	25.5	29.0
B.1.617.2 (Delta)	4.64 × 104	RNA copies/mL	1.8 × 108	2.1 × 107	2.3 × 106	2.8 × 105	3.8 × 104
		Ct value E gene	15.9	19.3	22.7	26.0	29.1

Ct: cycle threshold; NA: not available; E gene: envelope-protein gene E. * Estimated values based on the 1:1000 dilution step, due to invalid PCR results for the B.1.351 dilution steps 1:10,000 and 1:100,000 in saliva (consistency of the B.1.351 dilution series in saliva was verified in a separate PCR, repeated with the EDX SARS-CoV-2 standard (Exact diagnostics) after 24 h).