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. 2021 Sep 14;11(9):961. doi: 10.3390/life11090961

Figure 2.

Figure 2

TNFR1 and TNFR2 mRNA expression in the brain regions of TNFR2+/− and TNFR2−/− mice receiving peripheral LPS injections. (A) Total RNA was isolated from the cortex and hippocampus of TNFR2+/− and TNFR2−/− mice after the peripheral injection with Vehicle or LPS (0.5 mg/kg/day) for 7 days. The samples were subjected to QPCR analysis for the measurement of TNFR1. (B) Total RNA was isolated from cortex and hippocampus of TNFR2+/− mice receiving a daily peripheral injection with Vehicle or LPS (0.5 mg/kg/day) for 7 days, and then subjected to QPCR for the measurement of TNFR2 mRNA expression. (C) The cortical tissues were dissected from wildtype (WT), TNFR2+/− and TNFR2−/− mice after a 7-day injection with Vehicle or LPS (0.5 mg/kg/day). Total RNA was isolated and then subjected to QPCR for the measurement of TNFα mRNA expression. Data are presented as mean ± SEM (n = 3 animals per group). One dot indicated by the different symbol (◯, ●, □, ■, ▲, or △) represents an animal. * p < 0.05, ** p < 0.01, *** p < 0.001.