Table 1.
A list of primer sequences used for genotyping and Q-PCR analysis and sequences of shRNA against TNFR2.
| Gene | Forward (5′->3′) | Reverse (5′->3′) |
|---|---|---|
|
TNFR2 allele (t) |
AGCTCCAGGCACAAGGGCGGG | CCTCTCATGCTGTCCCGGAA |
| neomycin-resistant gene gene (neoR) | CACGGGTAGCCAACGCTATGTC | GCCCTGAATGAACTGCAGGACG |
| CyPA | CGTCTGCTTCGAGCTGTTTG | GTAAAATGCCCGCAAGTCAA |
| TNFα | ACCGTCAGCCGATTTGCTAT | CCGGACTCCGCAAAGTCTAA |
| TNFR1 | GCTTGTGTCCCCAAGGAAAG | GTCCTGGGGGTTTGTGACAT |
| TNFR2 | GTCGCGCTGGTCTTCGAACTG | GGTATACATGCTTGCCTCACAGTC |
| shRNA | Sequence | |
| sh-ctrl (Scramble) | AAAAGCTACACTATCGAGCAATTTTGGATCCAAAATTGCTCGATAGTGTAGC | |
| sh-mTNFR2-188 | AAAAGCCAGATCTCACAGGAATATTGGATCCAATATTCCTGTGAGATCTGGC | |
| sh-mTNFR2-220 | AAAAGCTCAGATGTGCTGTGCTATTGGATCCAATAGCACAGCACATCTGAGC | |