Skip to main content
. 2021 Sep 10;22(18):9821. doi: 10.3390/ijms22189821

Table 1.

Representative studies showing the effect of ROS on endothelial Ca2+ homeostasis.

ROS Mechanism of ROS Stimulation Dose of ROS or of ROS-Generating Enzymes ROS Scavenger Endothelial Cell Type Effect on Intracellular Ca2+ Homeostasis Reference
H2O2 Acute exposure 1-5-10 mM Not used CJVECs ICR and ECI [130]
H2O2 Acute exposure 100 µM Not used CPAECs ICR and ECI [131]
H2O2 Acute exposure 500 µM Not used SRLECs
HUVECs		 ICR [129]
H2O2 Acute exposure 100 µM-10 mM Not used HAECs ICR [139]
H2O2 Acute exposure 10 µM Not used HUVECs Not determined [79]
H2O2 Acute exposure 1 mM Not used ICR [140]
H2O2 Acute exposure 5 mM Cat, effect
DMSO, no effect		 MAECs
MesAECs		 ICI and ECI [138]
H2O2 Acute exposure 100 µM BAECs ICI [115]
H2O2 Acute exposure 10-100 µM Cat, effect
NAC, effect		 HUVECs Increases agonists-induced Ca2+ signaling [150]
H2O2 HX/XO 1 mM HX/2 mU/mL XO Cat, effect
SOD, no effect
O-phen, no effect		 SRLECs
HUVECs		 ICR [129]
H2O2 G/GO 10 mM G/2 mU/mL GO Cat, effect
SOD, no effect
O-phen, no effect		 SRLECs
HUVECs		 ICR [129]
H2O2 HX/XO 0.5 mM HX/50 mU/mL XO Cat, effect
SOD, no effect		 CPAECs ICR and ECI [128]
H2O2 G/GO 10 nM G/[GO] →10 nM H2O2/mL/min Not used HUVECs Not determined [79]
H2O2, O2•− and •OH HX/XO 2 mM HX/[XO] → O2- nM/mL/min Cat, effect
SOD, effect
O-phen, effect		 HUVECs ICI and ECI [79,135]
H2O2 and O2•− HX/XO 200 µM HX/20 mU/mL XO Cat, effect
SOD, effect 		MAECs
MesAECs		 ICI and ECI [138]
O2•− HX/XO 1 mM HX/150 mU/mL XO SOD, effect PAECs Increases agonist-induced ICI and SOCE [136]
H2O2, O2•− and •OH X/XO 200 µM HX/2 mU/mL XO Cat, effect
SOD, effect
O-phen and Def, effect		 PAECs ICI and ECI [137]

Abbreviations: BAECs: bovine aortic endothelial cells; CJVECs: canine jugular venous endothelial cells; CPAECs: calf pulmonary artery endothelial cells; Def: deferoxamine: DMSO: dimethyl sulfoxide; G/GO: glucose/glucose oxidase; HAECs: human aortic endothelial cells; ICR: intracellular Ca2+ release; ECI: extracellular Ca2+ influx; MAECs: mouse aortic endothelial cells; MesAECs: mesenteric artery endothelial cells; NAC: N-acetylcysteine; O-phenanthroline: O-phen; PAECs: porcine aortic endothelial cells; SRLECs: sinusoidal rat liver endothelial; X/XO: xanthine/xanthine oxidase. Def, DMSO and O-phen prevent •OH formation by inhibiting the Fentom reaction.