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. 2021 Sep 10;13(9):1448. doi: 10.3390/pharmaceutics13091448

Figure 1.

Figure 1

Cytotoxicity assay of 3D printed cyclic strain bioreactor with CHO-K1 and HeLa cells. MTT assay on CHO-K1 and HeLa cells seeded on coverslips at 24 h and 48 h of culture in the bioreactor chamber. The histograms report the mean percentage of viable cells compared to control (cells cultured in a standard plate, 100%) (a). Live and Dead assay at 24 h and 72 h on HeLa cells embedded in the 3D fibrin hydrogel of the 3D scaffold. The green signal, indicating viable cells, was quantified using ImageJ software and presented as fold change over T0 = 1 (b). Statistically significant differences are shown as ** = p ≤ 0.01 compared to T0. Scale bar = 100 μm.