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. 2021 Sep 10;13(9):1448. doi: 10.3390/pharmaceutics13091448

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Images of emulsion and derived PLGA-NCs obtained by SEE technology, particle size distribution, and hGDF-5 release profiles within the 3D environment. Optical microscope image of emulsion (a) and electronic microscope image (b) of carriers obtained after emulsion processing by SEE technology; size distribution data of PLGA carriers expressed as volume percentage (c); in vitro hGDF-5 release profile (ng/mL/day) monitored at 37 °C and 100 rpm by ELISA-based assay from 80 mg of carriers, as loaded in each construct (d); n = 2.