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. 2021 Sep 10;13(9):1448. doi: 10.3390/pharmaceutics13091448

Figure 8.

Figure 8

Gene expression profiles for cytokines by hWJ-MSCs cultured within scaffold assembled with PLGA-NCs for hGDF-5 controlled delivery. The mRNA levels of different pro-inflammatory (IL-6, TNF, IL-12A, IL-1β) and anti-inflammatory (IL-10, TGF-β1) cytokines were monitored. Relative quantification of each mRNA gene expression normalized to endogenous GAPDH (internal control) was calculated using the 2−ΔΔCt method and presented as fold change over hWJ-MSCs T0 = 1 (dashed line). Statistically significant differences are shown as “b” = p ≤ 0.001; “c” = p ≤ 0.005; “d” = p ≤ 0.001; compared to T0; n = 2 (biological replicates). The overexpression of pro-inflammatory factors (IL-6, TNF) was significant, together with the upregulation of anti-inflammatory ones (IL-10, TGFβ1).