Figure 2.

CRC-PDE culture cell viability. (a) Representative images of CRC-PDE culture at day 0 (immediately after processing of the tumour tissue) and after 4 weeks, stained with fluorescein diacetate (FDA, green) and Propidium Iodide (PI, red) for detection of live and dead cells, respectively. Scale bar: 500 µm. (b) Measurement of resazurin reduction capacity of the CRC-PDE cultures along time. Data are presented as the mean of fold change relatively to day 0 ± SD, calculated from 10 biological replicates. Statistical significance was evaluated by a mixed effects model for analysis of repeated measurements followed by a post-hoc Tukey’s multicomparison test. CRC-PDE, colorectal cancer patient-derived explants; ns, non-significant.