Table 2.
Genes and primers used in gene expression analysis. Sequence of sense (S) and antisense (A) primers used in the real-time RT-PCR of reference and target genes. Base pairs (bp).
| Related Function | Gene | Primer Sequence (5′-3′) | Product Size (bp) | GenBank ID |
|---|---|---|---|---|
| ECM component | Collagen I α1 (COL1A1) |
S: CCTGACGCACGGCCAAGAGG A: GGCAGGGCTCGGGTTTCCAC |
122 | NM_000088.3 |
| Collagen III α1 (COL3A1) |
S: GGCTACTGGGCCTGGTGGT A: CCACGTTCACCAGGGGCACC |
190 | NM_000090.3 | |
| Decorin (DCN) |
S: ATCTCAGCTTTGAGGGCTCC A: GCCTCTCTGTTGAAACGGTC |
146 | NM_001920.3 | |
| ECM turnover | Matrix metalloproteinase-1 (MMP-1) | S: TGTCAGGGGAGATCATCGGGAC A: TGGCCGAGTTATGAGCTGCA |
177 | NM_002421.3 |
| Tissue inhibitor of metalloproteinase-1 (TIMP-1) |
S: TTCCGACCTCGTCATCAGGG A: TAGACGAACCGGATGTCAGC |
144 | NM_003254.2 | |
| Wound Healing/Fibrogenic | α-Smooth muscle actin 2 (ACTA2) | S: TAAGACGGGAATCCTGTGAAGC A: TGTCCCATTCCCACCATCAC |
184 | NM_001141945.1 |
| Transforming growth factor-β1 (TGF-β1) | S: TGTCACCGGAGTTGTGCGGC A: GGCCGGTAGTGAACCCGTTG |
131 | NM_000660.4 | |
| Endothelin-1 (END) |
S: ACGGCGGGGAGAAACCCACT A: ACGGAACAACGTGCTCGGGA |
147 | NM_001955.4 | |
| Reference gene | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
S: TGC ACC ACC AAC TGC TTA GC A: AAG GGA CTT CCT GTA ACA A |
87 | NM_002046.3 |
| Beta-Actin (ACTBL2) |
S: CTG GAA CGG TGA AGG TGA CA A: AAG GGA CTT CCT GTA ACA A |
140 | NM_001101.3 | |
| 18S ribosomal RNA (18S rRNA) |
S: GTAACCCGTTGAACCCCATT A: CCATCCAATCGGTAGTAGCG |
151 | NR_146156.1 |