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. 2021 Sep 3;10(9):2307. doi: 10.3390/cells10092307

Figure 2.

Figure 2

Assessment of Affimers binding to EPAC1ΔDEP in the presence or absence of agonist and antagonist. Affimer binding to biotinylated EPAC1ΔDEP protein was determined by iQue assay, in the presence and absence of 10 µM 007 (EPAC1 agonist) or 10 µM CE3F4 (EPAC1 antagonist). Data were normalized to the median fluorescent intensity of the positive control Affimer (Affimer G12 against immobilized mIgG2b), which was set to 100%. Significant increases in binding were determined by one-way ANOVA. Relative differences in binding relative to EPAC1ΔDEP plus 10 µM 007, are indicated; * p < 0.05; ** p < 0.001 and *** p < 0.0001, respectively (n = 3). Results are presented as the means ± SD (n = 3). ns: Not significant.