Table 1.
Target genes and details of the primer sequences used for quantitative SYBR Green real-time PCR amplification. The GAPDH gene was used as the endogenous control gene.
| Target Gene |
GenBank Accession No |
Primer Sequence | [C] (nM) |
Efficiency (%) |
Slope | r2 | Amplicon Length (bp) |
|---|---|---|---|---|---|---|---|
| CAT-1 [22] | NM_001012613 | F: 5′-AGACGGGCTGCTGTTTAAGT-3′ R: 5′-ACCGTTAAAATACCGGCGTG-3′ |
300 | 100.6 | −3.30 | 0.99 | 131 |
|
IL-8 [23] |
NM_213867 | F: 5′-CCGTGTCAACATGACTTCCAA-3′ R: 5′-GCCTCACAGAGAGCTGCAGAA-3′ |
300 | 98.9 | −3.29 | 0.99 | 75 |
|
IL-6 [24] |
NM_214399 | F: 5′-GGCAAAAGGGAAAGAATCCAG-3′ R: 5′-CGTTCTGTGACTGCAGCTTATCC-3′ |
300 | 99.6 | −3.33 | 0.99 | 87 |
| TGF-β1 [25] | NM_214015 | F: 5′-AGGGCTACCATGCCAATTTCT-3′ R: 5′-CCGGGTTGTGCTGGTTGTACA-3′ |
300 | 106.3 | −3.18 | 0.98 | 102 |
| TNF-α [24] | NM_214022 | F: 5′-ACTGCACTTCGAGGTTATCGG-3′ R: 5′-GGCGACGGGCTTATCTGA-3′ |
300 | 98.5 | −3.36 | 0.99 | 118 |
|
IL-1α [26] |
NM_214029 | F: 5′-GCTCAAAACGAAGACGAACC-3′ R: 5′-TGATGGTTTTGGGTGTCTCA-3′ |
300 | 99.4 | −3.34 | 0.97 | 61 |
|
GAPDH (Primer Express) |
NM_001206359 | F: 5′-GGTGAAGGTCGGAGTGAACG-3′ R: 5′-GCCAGAGTTAAAAGCAGCCCT-3′ |
300 | 102.0 | −3.27 | 0.99 | 70 |
bp: base pairs; F: forward primer; R: reverse primer. CAT-1: cationic amino acid transporter-1; IL: interleukin; TGF-β1: transforming growth factor-beta 1; TNF-α: tumour necrosis factor-alpha; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.