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. 2021 Aug 24;9(9):1799. doi: 10.3390/microorganisms9091799

Figure 2.

Figure 2

(a) Time course of replication of baculovirus DNA during rAAV8-γSGC production in the dual baculovirus system with one baculovirus-encoding recombinant rAAV8-γSGC transgene inserted at the Tn7 site and the second baculovirus encoding cap8 gene at the egt locus and rep2 gene inserted at the Tn7 site. Total MOI was 0.1 (n = 2). Follow-up was performed for 96 h. Baculovirus DNA copy number, based on DNA Polymerase qPCR amplicon measurement, is expressed in number of copies per mL of cell culture. (b) Ratio of rAAV8-γSGC gene copy number to baculovirus DNA copy number, measured by qPCR of the baculovirus DNA polymerase gene amplicon, during rAAV8-γSGC production.