Figure 5.

Relative expression in arbitrary units (a.u.) of transcripts of phenylalanine ammonia lyase 1 (VvPAL1) (a,b), chalcone synthase 1 and 3 (VvCHS1 and VvCHS3) (c–f), and stilbene synthase 1 (VvSTS1) gene (g,h), in exocarp and seeds, from berries grown at two distinct light microclimates (LL and HL), and at three developmental stages (green, véraison, and mature), as determined by real-time qPCR. Expression levels are normalized to the mean expression of reference genes VvACT1 and VvGAPDH. Statistical analysis (two-way ANOVA, p ≤ 0.05, n = 3) was applied after data Log(X+1) transformation. Statistical notation: capital letters refer to differences between developmental stages for the same microclimate, and lowercase letters refer to differences between microclimates for each stage. When the letters are omitted, it means that the respective factor did not have a significant effect.