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Substitutions of artificial sequences for regions required for autonomous replication of ars2004M. (A) Region I of ars2004M was replaced with a synthetic (A/T)40, (AAAT/TTTA)10, (AAT/TTA)13, (AT/TA)20, or (AAAC/TTTG)10 fragment. Transformation efficiencies relative to that of pARS2004M after 3 days of incubation are presented. (B) Each region or all three regions were replaced with (A/T)40 or (AT/TA)20. A/T stretches, but not AT alternates, function like the natural essential regions.
