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Autonomous replication of ars2004M without spacer regions. Transformation efficiencies with derivatives of pYC11 carrying combinations of regions I, II, and III and (A/T)40 fragments without spacer regions of ars2004M were measured after 4 days of incubation. Fragments inserted into the vector are schematically shown. Regions I, II, and III were joined in the natural order and direction. The boxes labeled T40 represent (A/T)40 fragments inserted in the opposite orientation.
