Figure 3.

Tg2576 mice exhibit reduced ChAT⁺ area in pre-synaptic terminals of TA muscles. (A) Representative images of TA sections, stained with antibodies anti-NFL (green) and anti-ChAT (red), from Tg2576 and wild type mice. Sections were counterstained with DAPI in order to detect cell nuclei. White arrows point to pre-synaptic area, positive for both markers. Scale bar = 50 µm. (B) Histogram reporting the average area of ChAT signal in pre-synaptic surface, identified by the presence of both NFL and ChAT signals, in TA sections of Tg2576 and wild type mice (n = 5 mice per genotype). Unpaired t test was used for comparison (* = p < 0.05).