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. 2021 Sep 8;26(18):5462. doi: 10.3390/molecules26185462

Figure 5.

Figure 5

Establishment of cells for GPI-TA purification. (A,B) K562 PIGK-KO (K562K) cells and K562K cells stably expressing PIGK-StrepII-GST were lysed, and PIGK-StrepII-GST was detected by the anti-GST antibody (A). The surface expression of CD55 in these cells was analyzed using flow cytometry (B). (C,D) K562 PIGK-KO (K562K) cells and K562K cells stably expressing PIGK-StrepII were lysed, and PIGK-StrepII was detected by the anti-StrepII antibody (C). Surface expression of CD55 in those cells was analyzed using flow cytometry (D). (E,F) PIGK-StrepII-GST (E) and PIGK-StrepII (F) samples were prepared under reducing (sample buffer with dithiothreitol (+DTT)) and nonreducing (sample buffer without dithiothreitol (−DTT)) conditions. Both PIGK-StrepII-GST and PIGK-StrepII were detected at higher molecular weights under the nonreducing condition, indicating the formation of intermolecular disulfide bonds with PIGT.