Figure 5.

Change in the percentage of sperm with cytoplasmic droplets during storage at 17 °C and subsequent incubation at 38 °C. Semen samples from individual boars (n = 11) were scored for the presence of spermatozoa with proximal or distal cytoplasmic droplets. (A) Two hundred cells were evaluated after 24 h and 96 h storage at 17 °C in the BTS extender. Cells were fixated in fixating buffer prior to evaluation. (B) Similarly, cells were evaluated after 24 h storage prior to or after 120 min incubation at 38 °C. No significant difference was observed due to storage or incubation time (p > 0.05). Incubation at 38 °C after 96 h storage had also no effect (data not shown).