Table 1.
Accuracy of all potential QIs for discrimination of TTC ≤ 1 h and 2 h in validation samples of healthy volunteers.
| Potential QIs | Median Opt. Cutoff | Specificity n+/n > 1 h | Sensitivity 1-(n-/n) < 1 h | Median Opt. Cutoff | Specificity n+/n > 2 h | Sensitivity 1-(n-/n) < 2 h |
|---|---|---|---|---|---|---|
| Purines and Pyrimidines in serum of validation sample 1 | ||||||
| Guanosine # | 0.50 µmol/L | 100% | 80% | 0.28 µmol/L | 100% | 83% |
| Hypoxanthine | 32 µmol/L | 75% | 83% | 37 µmol/L | 86% | 80% |
| Xanthine | 1.5 µmol/L | 75% | 67% | 1.6 µmol/L | 80% | 67% |
| Inosine # | 1.25 µmol/L | 86% | 75% | 1.13 µmol/L | 100% | 67% |
| Dihydroorotic acid | 32 µmol/L | 83% | 75% | 38 µmol/L | 94% | 75% |
| XG-ratio | 0.61 | 80% | 80% | 0.93 | 89% | 75% |
| HI-ratio | 1.41 | 83% | 86% | 1.57 | 78% | 100% |
| HG-ratio | 2.06 | 100% | 80% | 2.25 | 88% | 75% |
| XI-ratio | 0.14 | 78% | 83% | 0.24 | 73% | 100% |
| Eicosanoids in serum of validation sample 1 | ||||||
| Tetranor-12(S)-HETE | - | - | - | 0.45 ng/mL | 88% | 90% |
| 12(S)-HEPE | - | - | - | 8.17 ng/mL | 100% | 100% |
| (±)13-HODE | - | - | - | 6.83 ng/mL | 83% | 100% |
| (±)9-HODE | - | - | - | 5.31 ng/mL | 62% | 67% |
| 15(S)-HETE | - | - | - | 3.36 ng/mL | 100% | 100% |
| 11-HETE | - | - | - | 3.84 ng/mL | 86% | 75% |
| 8(S)-HETE | - | - | - | 2.22 ng/mL | 100% | 100% |
| 12-HETE | - | - | - | 15.14 ng/mL | 100% | 100% |
| 5-HETE | - | - | - | 2.63 ng/mL | 60% | 60% |
| 12-oxo-ETE | - | - | - | 2.62 ng/mL | 100% | 100% |
| Amino acids in serum of validation sample 2 | ||||||
| Arginine # | 86.15 µmol/L | 64% | 67% | 83.4 µmol/L | 70% | 65% |
| Ornithine | 91.24 µmol/L | 67% | 69% | 106.31 µmol/L | 74% | 67% |
| OA-ratio | 0.01 | 78% | 77% | 0.05 | 75% | 75% |
| Purines and Pyrimidines in EDTA plasma of validation sample 1 | ||||||
| Hypoxanthine | 12 µmol/L | 50% | 71% | 11.5 µmol/L | 80% | 75% |
| Inosine # | 0.5 µmol/L | 100% | 33% | 0.5 µmol/L | 89% | 0% |
| Dihydroorotic acid | 12.2 µmol/L | 50% | 75% | 12.65 µmol/L | 67% | 71% |
| HI-ratio | 1.54 | 40% | 75% | 1.51 | 67% | 80% |
| Eicosanoids in EDTA plasma of validation sample 1 | ||||||
| (±)13-HODE | - | - | - | 5.45 ng/mL | 78% | 67% |
| (±)9-HODE | - | - | - | 2.49 ng/mL | 88% | 67% |
| 5-HETE | - | - | - | 0.41 ng/mL | 67% | 50% |
| Amino acids in EDTA plasma of validation sample 2 | ||||||
| Arginine # | 51.24 µmol/L | 73% | 69% | 46.62 µmol/L | 75% | 65% |
| Ornithine | 87.94 µmol/L | 71% | 67% | 110.38 µmol/L | 88% | 67% |
| OA-ratio | 0.23 | 85% | 73% | 0.3 | 78% | 83% |
The table displays the calculated median optimal cutoffs for all identified potential QIs in validation sample 1 and 2 in serum and EDTA plasma. # Cutoff values for arginine, inosine and guanosine (concentration decreases over time) are calculated for a TTC ≥ 1 or 2 h, respectively. QIs with the highest sensitivity and specificity are highlighted in bold.