Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Reagents for preparation of SBF (pH 7.30–7.40, 2000 mL).
| Order | Reagent | Amount |
|---|---|---|
| 1 | NaCl | 16.072 g |
| 2 | NaHCO3 | 0.704 g |
| 3 | KCl | 0.450 g |
| 4 | K2HPO4·3H2O | 0.460 g |
| 5 | MgCl2·6H2O | 0.622 g |
| 6 | 1.0 M-HCl | 6 mL |
| 7 | CaCl2 | 0.586 g |
| 8 | Na2SO4 | 0.144 g |
| 9 | (CH2OH)3CNH2(TRIS) a | 12.126 g |
a TRIS: tris(hydroxymethyl)amiomethne.
