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. 2021 Sep 14;10(9):2420. doi: 10.3390/cells10092420

Figure 4.

Figure 4

Array to evaluate the changes in SMUP-Cell protein secretion under inflammatory conditions. (A) Array analysis using supernatants from SMUP-Cell cultured alone versus SMUP-Cells co-cultured with LPS-exposed Raw 264.7 cells. Secreted proteins were characterized using a protein array (55-spot, n = 1). Eight proteins in the membrane were upregulated in the co-culture group (squares). (B) Quantification of optical intensity of anti-inflammatory factors showed upregulated protein secretion in the co-culture relative to that in the culture of SMUP-Cells. Protein levels were evaluated according to fold increase, with data normalized to levels from a culture of SMUP-Cells alone (set to 1). (C) To confirm the upregulation of PTX-3, its level was analyzed by ELISA in supernatants from cultures of SMUP-Cells alone and co-cultures in four different lots. Data represent mean ± SD (n = 3 per group). *** p < 0.01. R, Raw 264.7 cells; L, LPS treatment.