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. 2021 Sep 16;22(18):10025. doi: 10.3390/ijms221810025

Figure 2.

Figure 2

Binding of the RNA-PPRH to its ssDNA or dsDNA target sequence. (A) Gel-shift assays using 300 ng of a 5′-FAM labeled ssDNA probe (ssDNA-FAM). The unlabeled oligonucleotides present in each binding reaction are indicated. Lane 1, ssDNA-FAM probe alone; lane 2, ssDNA-FAM plus HpsPr-C-RNA (600 ng); lane 3, ssDNA-FAM plus HpsPr-C-DNA (600 ng); lane 4, ssDNA-FAM plus HpSC6 (600 ng); lane 5, ssDNA-FAM plus HpsPr-C-RNA (300 ng); lane 6, ssDNA-FAM plus HpsPr-C-RNA (600 ng); lane 7, ssDNA-FAM plus HpsPr-C-RNA (1200 ng). (B) Gel-shift assays using 200 ng, 100 ng, or 50 ng of a 5′-FAM labeled dsDNA probe (dsDNA-FAM). The unlabeled oligonucleotides present in each binding reaction are indicated. Lane 1, dsDNA-FAM probe alone (200 ng); lane 2, dsDNA-FAM (200 ng) plus HpsPr-C-RNA (200 ng); lane 3, dsDNA-FAM probe alone (100 ng); lane 4, dsDNA-FAM (100 ng) plus HpsPr-C-RNA (200 ng); lane 5, dsDNA-FAM (100 ng) plus HpsPr-C-RNA (600 ng); lane 6, dsDNA-FAM (100 ng) plus HpsPr-C-RNA (1200 ng); lane 7, dsDNA-FAM probe alone (50 ng); lane 8, dsDNA-FAM (50 ng) plus HpsPr-C-RNA (200 ng); lane 9, dsDNA-FAM (50 ng) plus HpsPr-C-RNA (600 ng); lane 10, dsDNA-FAM (50 ng) plus HpsPr-C-RNA (1200 ng); lane 11, dsDNA-FAM (200 ng) plus HpsPr-C-DNA (200 ng); lane 12, dsDNA-FAM (200 ng) plus HpSC6 (200 ng); Lane 13, dsDNA-FAM probe alone (200 ng).